产品货号：
Z13961
中文名称：
DHFR抗体
英文名称：
Anti-DHFR Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-DHFR Antibody |
| 指标别名 | DHFR;DHFRP1;dihydrofolate reductase |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,FCM,ICC/IF |
| 基因名称 | DHFR |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human DHFR recombinant protein (Position:V2-D187).Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR. |
| 计算分子量 | 21KD |
| 实际分子量 | 22KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Dihydrofolate reductase,or DHFR,is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid,using NADPH as electron donor,which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry.In humans,the DHFR enzyme is encoded by the DHFR gene.It is found in the q11→q22 region of chromosome 5.What's more,DHFR belongs to the dihydrofolate reductase family,and it converts dihydrofolate into tetrahydrofolate,a methyl group shuttle required for the de novo synthesis of purines,thymidylic acid,and certain amino acids.DHFR is the key enzyme in folate metabolism.In addition,DHFR catalyzes an essential reaction for de novo glycine and purine synthesis,and for DNA precursor synthesis. |
| Uniprot ID | P00374 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | dihydrofolate reductase |
| 蛋白名全称 | Dihydrofolate reductase |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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