产品货号：
Z11601
中文名称：
B3GNT8抗体
英文名称：
Anti-B3GNT8 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-B3GNT8 Antibody |
| 指标别名 | UDP-GlcNAc:betaGal beta-1;3-N-acetylglucosaminyltransferase 8;B3GALT7;BGALT15;beta3Gn-T8;B3GNT8 |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC |
| 基因名称 | B3GNT8 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human B3GNT8 (360-397aa ADRTADHCAFRNLLLVRPLGPQASIRLWKQLQDPRLQC),different from the related mouse sequence by sixteen amino acids. |
| 实际分子量 | 43KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | B3GNT8 is a galactosyltransferase involved in the synthesis of poly-N-acetyllactosamine (polyLacNAc),a linear chain of repeating LacNAc units made up of galactose (Gal) and N-acetylglucosamine (GlcNAc) with the structure (Gal-beta-1-4-GlcNAc-beta-1-3)n.By genomic sequence analysis,the B3GNT8 gene is mapped to chromosome 19q13.2.It was showed that a soluble form of B3GNT8 overexpressed by transfected HEK293 cells selectively transferred GlcNAc from UDP-GlcNAc to the nonreducing terminus of Gal-beta-1-4-GlcNAc-alpha-p-nitrophenyl phosphate and to lactoside-alpha-benzoyl.It did not utilize keratan sulfates or polylactosamine oligosaccharide as substrate.B3GNT8 activity required Mn(2+) and showed less efficiency with Co(2+).The pHoptimum was between 7 and 7.5.B3GNT8 also transferred GlcNAc onto alpha-1-acid glycoprotein and ovomucoid,which possess tetraantennary complex type and pentaantennary complex type N-glycans.With a tetraantennary N-glycan substrate,B3GNT8 appeared to prefer the beta-1-2 branch over the beta-1-6 branch.When overexpressed in HCT15 human colon cancer cells,B3GNT8 increased cell surface expression of both polyLacNAc and beta-1-6-branched N-glycans. |
| Uniprot ID | Q7Z7M8 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 |
| 蛋白名全称 | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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