产品货号：
Z11286
中文名称：
APC3/CDC27抗体
英文名称：
Anti-APC3/CDC27 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-APC3/CDC27 Antibody |
| 指标别名 | ANAPC3;APC3;CDC27;CDC27 homolog;CDC27;APC3;CDC27Hs;D0S1430E;D17S978E;H NUC;HNUC |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,FCM,ELISA |
| 基因名称 | CDC27 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human CDC27 recombinant protein (Position:H609-F824). |
| 实际分子量 | 92KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene.The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27,and the gene product of Schizosaccharomyces pombe nuc 2.This protein is a component of the anaphase-promoting complex (APC),which is composed of eight protein subunits and is highly conserved in eukaryotic cells.This complex catalyzes the formation of cyclin B-ubiquitin conjugate,which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins.The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats,which are important for protein-protein interactions.This protein was shown to interact with mitotic checkpoint proteins including Mad2,p55CDC and BUBR1,and it may thus be involved in controlling the timing of mitosis.Alternative splicing of this gene results in multiple transcript variants.Related pseudogenes have been identified on chromosomes 2,22 and Y. |
| Uniprot ID | P30260 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot. |
| 基因名全称 | cell division cycle 27 |
| 蛋白名全称 | Cell division cycle protein 27 homolog |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells Enzyme linked immunosorbent assay (ELISA) 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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