产品货号：
Z10603
中文名称：
AKT1/2/3抗体
英文名称：
Anti-AKT1/2/3 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-AKT1/2/3 Antibody |
| 指标别名 | AKT;AKT1;PKB;PKB ALPHA;PRKBA;Protein kinase B;Proto oncogene c Akt;RAC;RAC ALPHA;RAC PK alpha;AKT2;PKB beta;PKBB;PKBBETA;PRKBB;Protein kinase Akt 2;Protein kinase B beta;RAC BETA;RAC PK beta;AKT3;PKB GAMMA;PKBG;PRKBG;Protein kinase Akt 3;Protein kinase B gamma;RAC gamma;RAC PK gamma;STK 2 |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,ICC/IF,FCM,ELISA |
| 基因名称 | AKT1/AKT2/AKT3 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human AKT1,2,3 recombinant protein (Position:E17-A477). |
| 实际分子量 | 60KD |
| 成分 | 500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene.This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha,beta,and gamma.These highly similar AKT proteins all have an N-terminal pleckstrin homology domain,a serine/threonine-specific kinase domain and a C-terminal regulatory domain.These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K).AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases,G-protein coupled receptors,and integrin-linked kinase.These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation,survival,metabolism,and angiogenesis in both normal and malignant cells.AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K.Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.Phosphorylation of additional residues also occurs,for example,in response to insulin growth factor-1 and epidermal growth factor.Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3.The PI3K/AKT signalling pathway is crucial for tumor cell survival.Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery.AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway,in addition to responding to extracellular signals from growth factors and cytokines,is disregulated in many cancers.Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6,and breast,colorectal,and ovarian cancers.Multiple alternatively spliced transcript variants have been found for this gene. |
| Uniprot ID | P31749/P31751/Q9Y243 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for ICC. |
| 基因名全称 | AKT serine/threonine kinase 1,2,3 |
| 蛋白名全称 | RAC-alpha/beta/gamma serine/threonine-protein kinase |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells ELISA：1:100～1000 |
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