产品货号：
Z10478
中文名称：
ADO抗体
英文名称：
Anti-ADO Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-ADO Antibody |
| 指标别名 | 2 aminoethanethiol dioxygenase;ADO;C10orf22;Cysteamine dioxygenase |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,IHC-F,ICC,ICC/IF,FCM |
| 基因名称 | ADO |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human ADO recombinant protein (Position:E49-E261).Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO. |
| 实际分子量 | 30KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO).CDO adds 2 oxygen atoms to free cysteine,whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine.It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine.Recombinant Ado was shown to bind iron.Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine.Similar results were found with human ADO.When endogenous expression of ADO was reduced by RNA-mediated interference,hypotaurine production decreased.It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity. |
| Uniprot ID | Q96SZ5 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | 2-aminoethanethiol dioxygenase |
| 蛋白名全称 | 2-aminoethanethiol dioxygenase |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunohistochemistry in frozen section (IHC-F) 1:50～400 Immunocytochemistry in fixed cells (ICC) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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