产品目录

本品为来源于兔的抗Ras蛋白的多克隆抗体。
宿主：兔
克隆性：多克隆抗体
用途：WB,FC, IHC,ICC, IF
交叉反应性：人，小鼠，大鼠
分子量：21KDa
推荐稀释比例：WB(1:1000),IF(1:200),IHC(1:200),ICC(1:200),FC(1:100-1:200)

About this Antibody
Name	Ras Rabbit Polyclonal Antibody
Category	Polyclonal antibody(pAb); Primary antibody
Isotype	IgG
Purification	Peptide affinity purified
About the Immunogen
Immunogen	This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to H-Ras, N-Ras and K-Ras.
Gene ID	4893/3265/3845
SwissProt	P01111/P01112/P01116
Synonyms	GTPase NRas; Transforming protein N-Ras; NRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; HRAS; HRAS1; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; KRAS; KRAS2; RASK2
Category	-
Background	Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H-Ras, N-Ras and K-Ras.

注意事项：
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
2. 回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。

储存条件：-20℃，有效期一年。
相关搜索：兔抗Ras抗体，Ras Rabbit Polyclonal Antibody