产品货号：
M04339
中文名称：
calcineurin抑制剂(Cyclosporin A)
英文名称：
Cyclosporin A
产品规格：
1mL(10mM)|100mg|200mg|500mg
发货周期：
1～3天
产品价格：
询价
Cyclosporin A是一种免疫抑制剂，能与亲环素结合，抑制calcineurin活性的IC50值为7 nM。Cyclosporin A也抑制CD11a/CD18粘附分子。
生物活性
化学数据
试验方法
溶剂及溶解度
相关搜索：calcineurin抑制剂(Cyclosporin A)，Cyclosporine，Ciclosporin，环孢霉素A，环孢素A，环孢多肽A，环孢灵，赛斯平，环孢素，CsA，59865-13-3
生物活性
| 产品描述 | Cyclosporin A is an immunosuppressant which binds to the cyclophilin and inhibits phosphatase activity of calcineurin with an IC50 of 5 nM.Cyclosporin A also inhibits CD11a/CD18 adhesion. |
| 靶点及IC50 | IC50:7 nM (calcineurin) |
| 体外活性 | Cyclosporin A is able to bind with the cyclophilin in T cells.Cyclosporin A works by forming a Cyclophilin-Cyclosporin A complex to inhibit calcineurin.Cyclosporin A inhibits calcineurin in stimulated cells with an IC50 value of 7 nM.Cyclosporin A suppresses the nuclear translocation of NF-AT.Cyclosporin A shows an effect on mitochondria via preventing the MTP from opening with an IC50 of 39 nM. |
| 体内活性 | Cyclosporin A has immunosuppressive activity,and is active via parenteral and p.o.administration in mice,rat and guinea pigs.Cyclosporin A can be used in organ transplantation to prevent rejection. |
化学数据
| CAS号 | 59865-13-3 |
| 分子式 | C62H111N11O12 |
| 中文名称 | 环孢霉素A；环孢素A；环孢多肽A；环孢灵；赛斯平；环孢素 |
| 分子量 | 1202.61 |
| SMILES | C/C=C/C[C@H]([C@@H](O)[C@@]1(N(C)C([C@]([H])(C(C)C)N(C)C([C@H](CC(C)C)N(C)C([C@]([H])(CC(C)C)N(C)C([C@@H](C)NC([C@H](C)NC([C@@H](N(C([C@@]([H])(NC([C@@H](N(C(CN(C([C@@H](NC1=O)CC)=O)C)=O)C)CC(C)C)=O)C(C)C)=O)C)CC(C)C)=O)=O)=O)=O)=O)=O)[H])C |
| 结构式 |  |
| 纯度 | 99.85% |
| 保存 | 4℃,protect from light *In solvent:-80℃,6 months;-20℃,1 month (protect from light) |
试验方法
| 细胞实验 | Immunosuppressive agents are dissolved in ethanol at concentrations 1000-fold more than the concentration desired for cell treatments.Cells (106) are suspended in 1mL of complete medium in microcentrifuge tubes;1μL of ethanol or of the ethanolic solution of Cyclosporin A is added,and the cells are incubated at 37℃ for 1 hr.Cells are washed twice with 1mL of PBS on ice and lysed in 50μL of hypotonic buffer containing 50mM Tris (pH7.5);0.1mM EGTA;1mM EDTA;0.5mM dithiothreitol;and 50μg of phenylmethylsulfonyl fluoride,50μg of soybean trypsin inhibitor,5μg of leupeptin,and 5μg of Kiker 52G per mL.Lysates are subjected to three cycles of freezing in liquid nitrogen followed by thawing at 30℃ and then are centrifuged at 4℃ for 10min at 12000×g. |
| 动物实验 | Rats are immunized on day 0 i.p.with 0 5mL and mice i.v.with 0 2mL of a 10% suspension of washed sheep erythrocytes (SE).To elicit a secondary response,mice are boosted 8-11 weeks after the primary immunization with an i.v.injection of 0～2mL of 0 25% washed SE (107 cells).For prolonged treatment the animals receive on the average 45mg/kg cyclosporin A daily in the food during 13 weeks.These rats are immunized 5 days before killing. |
溶剂及溶解度
| In Vitro | DMSO：62.5mg/mL(51.97mM;Need ultrasonic) H2O：＜0.1mg/mL (insoluble)
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80℃,6 months;-20℃,1 month (protect from light)。-80℃储存时，请在6个月内使用，-20℃储存时，请在1个月内使用。 | ||||||||||||||||
| In Vivo | 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。
*以上所有助溶剂都可在我公司选购 |
相关搜索：calcineurin抑制剂(Cyclosporin A)，Cyclosporine，Ciclosporin，环孢霉素A，环孢素A，环孢多肽A，环孢灵，赛斯平，环孢素，CsA，59865-13-3